Estimate PCR annealing temperature from primer and target melting temperatures.
Annealing Temperature Calculator
How to use this calculator
Choose the temperature unit independently for both melting temperatures and for the result.
- Choose the calculation mode and units, when available.
- Enter values using the localized examples in the placeholders.
- Select Calculate and review the result, steps, and SVG visualization when provided.
Formula and method
Estimate PCR annealing temperature from primer and target melting temperatures.
Worked example
The placeholders provide a reproducible example. Enter them to verify the result before using your own data.
Assumptions and limitations
Values must be finite and use compatible units. Displayed decimals are rounded, while calculations retain full precision.
Frequently asked questions
What does this calculator do?
Estimate PCR annealing temperature from primer and target melting temperatures.
Why do fields appear or disappear?
Dependent fields follow the selected method, shape, ingredient, or unit mode.
Are all visible formulas semantic?
Yes. Formulas and structured results use native MathML, with text retained only for labels and copying.